Sentence examples for buffer before being resuspended from inspiring English sources

The cell pellet was then washed three times with 1.5 ml TES buffer before being resuspended in 1 ml of osmotic lysis buffer (50 mM Tris-Cl pH 7.5, 10 mM MgSO4, 0.8 M NaCl).

Cells were washed in MACS buffer before being resuspended at 30 × 10 cells per mL in the same buffer for sorting.

Beads were pelleted and washed four times in binding buffer before being resuspended in 50 μL of 1 × SB, boiled for 10 minutes, and analyzed by SDS-PAGE as described in the section above.

Beads were washed four times in ice-cold lysis buffer before being resuspended in 30  μl of 1 × gel-loading buffer (50 mM Tris-HCl (pH 6.8), 100 mM DTT, 2% SDS, 10% glycerol and bromophenol blue).

Starting with a maximum of 8 × 10 cells, they were pelleted by centrifugation (600× g for 2 min) and washed twice in KK2 buffer before being resuspended at 2 × 10 cells/ml in KK2 buffer and starved for 4 6 hr at 22°C by shaking at 180 rpm.

The cryopreserved purified microfilariae were thawed, and then rinsed twice in PBS and once in 1× DNase I Buffer (Roche) before being resuspended in 200 μL of DNase I Buffer containing 100 U of DNase I for 1 h at 37°C to remove any free human DNA.

Lysates were subjected to 15,000×g centrifugation for 15 min, and pellets were washed with 10 mM sodium phosphate buffer (pH7.5) containing 2%NP-40NP-40oresuspendedsuspended in 10 mM sodium phosphate buffer (pH7.5) and quantified again in triplicate using the Bradford assay.

Lymph node ex vivo or in vitro stimulated cells were washed with PBS before being resuspended in RLT buffer (Qiagen).

The isolated DNA was allowed to air-dry before being resuspended in TE buffer (10 mM Tris-HCl (pH 7.4) and 0.5 mM EDTA).

Briefly, 5  μL of the appropriate monoclonal antibody was incubated with 100  μL of splenic cells (1 × 10/mL) for 15 min in the dark before being resuspended in phosphate buffer saline (pH7.4 PBS) and before being analyzed.

Proteins were precipitated with 12% (v/v) trichloroacetic acid for 30 min on ice, pelleted at 16 000  g, rinsed twice with ice cold acetone before being resuspended in Laemmli Buffer (50 mM Tris-chloride pH 6.8, 100 mM dithiothreitol, 2% (v/v) sodium dodecyl sulfate, 10% (v/v) glycerol).