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Using Gooding's method [27], the upper leaves of Nicotiana tabacum L. inoculated with TMV were selected and ground in a phosphate buffer before being filtered through a double-layer pledget.
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The buffer solution was filtered through a 0.45 μm regenerated cellulose membrane filter.
The buffer was filtered through 0.45 μm filter before it was transferred to the inlet/outlet vials.
The sodium dihydrogen phosphate buffer was filtered through filters of 0.02-µm pore size (Whatman, Dassel, Germany) before use.
All buffers used were filtered through 0.2 µm nylon membrane filters (Nalgene, Rochester, NY).
All buffers were filtered through sterile 0.22 µm filter before use.
Buffers were filtered through a 0.22-μm filter before usage to remove any PK9119 precipitates.
All samples and buffers were filtered through a 0.2 μm filter (Millipore) before analysis.
Water (18 MΩ) was obtained from a Millipore Milli-Q water purification system, and all buffers were filtered through 0.2 μm nylon filter immediately before use.
All buffers were filtered through a 0.45-μm filter (Millipore, Tokyo, Japan) before the experiments.
All buffers were filtered through a 0.22 μm filter and autoclaved before use.
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