Sentence examples for buffer before being stained from inspiring English sources

As described previously[ 16], sections 4 μm in diameter were dried, deparaffinised, rehydrated and treated in a microwave for two rounds of five minutes in citrate buffer before being stained in a Techmate 500 (DAKO, Copenhagen, Denmark) with a polyclonal anti-HMG-CoAR antibody (Catalog # 07-457, Upstate) diluted 1 250.

Tissues were subjected to 20 min steam-heat-induced epitope retrieval in presence of 10 m M sodium citrate buffer (pH 5.6 6.0) before being stained with either 1  μg ml−1 of IgG or 4.0  μg ml−1 F ab) anti-L523S rabbit polyclonal antibody.

On day 15, the colonies were washed with cold phosphate buffer saline and fixed with −20°C cold methanol before being stained with 0.1% Coomassie Brilliant Blue.

Slides were then placed into neutralising buffer (0.4 M Tris, pH 7.5) for 2 × 10 min, before DNA was stained using SYBRgold (1 : 10 000 in TE buffer: 10 m M Tris, 1 m M EDTA, pH 7.5).

Thereafter, the cells are washed once with staining buffer before being incubated in staining buffer supplemented with 5% FBS on ice until sorting is completed.

Following addition of 400 μl binding buffer for a final volume of 500 μl the cells were stained with PI (0.6 μg/ml) immediately before measurement.

For cytoplasmic intracellular staining (Bcl-2), cells were stained for surface antibodies then fixed in IC fixation buffer before permeabilisation with perm buffer (eBioscience).

Cells were stained for 20 min at 4 °C in MACS buffer and washed in the same buffer before acquisition.

Other Israeli leaders had been stained by corruption before.

Foxp3 was stained using the Foxp3 Staining Buffer Set (eBioscience).

For intracellular staining of Foxp3, cells were stained using a Foxp3 Staining Buffer set (eBiosciences).