Sentence examples for buffer before being washed from inspiring English sources

Sections were fixed for 10 minutes in cold 4% paraformaldehyde in 0.1 M phosphate buffer before being washed in Depc-PBS (diethylpyrocarbonate treated phosphate buffered saline), incubated in acetylation solution for 10 minutes and permeabilised by incubation in 1% Triton X-100 (Sigma) for 5 minutes (human tissue) or 30 minutes (mouse tissue).

Afterwards, the membrane was incubated with primary antibody in blocking buffer overnight at 4°C before being washed twice with TBST (0.1% Tween in TBS) and incubated with the appropriate secondary antibody in blocking buffer for 1 hour at room temperature.

The slides were then left to stand for 10 minutes in buffer at room temperature before being washed thoroughly in tap water.

Sections were fixed in 4%paraformaldehyde 0.1M phosphate buffer (pH7.4) for 5 minutes before being washed with 6 changes of PBS (pH 7.4) over 20 minutes.

After three 3-min washes in PBS/Tween 20 buffer (wash buffer), sections were incubated in 1% glycine for 30 min to neutralise acidic groups before being washed again in the same wash buffer.

At the end of the treatments, cells were incubated in culture medium with 0.5 μM MitoSox probe at 37°C for 15 min before being washed with Hanks buffer (20 mM HEPES, pH 7.2, 10 mM glucose, 118 mM NaCl, 4.6 mM KCl and 1 mM CaCl2).

The incubation was terminated by 10-fold dilution with ice-cold PBS buffer before the cells were washed and submitted to FACS analysis.

All primary antibodies were diluted in 5% bovine serum albumin (BSA)/PBS buffer and incubated with the sections before they were washed three times with PBS and then incubated with HRP-labeled secondary antibody (1 200 dilution; KPL, Gaithersburg, MD, USA) for 50 minutes at room temperature.

The injection phase was 120 seconds followed by 60 seconds of buffer flow before the cells were washed at high flow rate.

Cells were washed three times in barcode-cytometry buffer and fixed in 1% paraformaldehyde (PFA) for 0.5 24 h before they were washed twice and resuspended in barcode-cytometry buffer.

Immediately before use, plates were washed once with HBSS buffer.