Sentence examples for buffer before being measured from inspiring English sources

Samples were then spun for 10 min in a microcentrifuge to pellet proteins, and supernatant was diluted 1 20 in buffer before being measured.

Xanthine oxidase (XO) (20 μL), diluted in buffer, was then added and the cuvette inverted to mix before being measured for three minutes at 550 nm.

The scattering from the buffer alone was measured before and after each sample measurement and the average of the scattering before and after each sample was used for background subtraction.

Lysis buffer temperature was measured with a thermometer immediately before buffer was poured on the scWestern surface.

The pH of the buffer was measured after dialysis.

The optical parameters (i.e., refractive index of the dispersion and the buffer solution) and the conductivity were measured before the DLS measurement.

After 5x washes with PBST, 200 uL of nitrophenyl phosphate substrate in diethanolamine buffer was added to each well and the plates incubated for 40 min at room temperature in the dark before absorbance was measured at 405 nm.

For quantitative analysis, HeLa, HepG2, and NIH 3T3 cells were treated as described above and permeabilized with cell lysis buffer (100 mL) before luciferase activity was measured in terms of relative light units (RLU) according to the manufacturer's instructions (Promega).

Wealth before colonization was measured in cows.

The mucus plume was allowed to grow for 45 min, the loose mucus was removed and the thickness was measured before the apical buffer was replaced with the same buffer containing 3% DSS or 3% Dextran.

The protein samples were incubated in buffers containing 0, 1, 2, 3, 5, 7, or 9 M urea for 18 h at 4 °C before the fluorescence was measured.