Sentence examples for buffer before being separated from inspiring English sources

Equal amounts of proteins (10  μg per condition) were boiled for 10 min in loading buffer before being separated on 15% SDS-PAGE gels.

They stole a kiss before being separated.

Snap frozen left ventricles from WT and CXCR5−/− mice were homogenized in cell lysis buffer and equal amounts of protein being separated from each sample by SDS-PAGE (10%) before transferred to polyvinylidene fluoride (PVDF) membranes.

For immunoblotting, cells were lysed in RIPA buffer and lysates were separated on polyacrylamide gels before transfer to nitrocellulose membranes.

Reactions were terminated by adding 5×SDS loading buffer and samples were separated on SDS PAGEs.

The proteins eluted with boiled SDS-PAGE sample buffer were separated by SDS-PAGE.

After five washings in lysis buffer, the beads were boiled in Laemmli buffer, and the proteins were separated by SDS-PAGE.

For each sample, 0.5 µg of digested peptides are loaded onto the trap column at 1 µl/min and desalted with 10 µl of Buffer A. The peptides are separated through a 100 min linear gradient from 100% Buffer A to 30% Buffer B and then going up to 100% Buffer B in 1 min, followed by a 3-min 100% Buffer B wash before returning to 100% Buffer A in 2 min and maintaining at 100% Buffer A for 4 min.

The cells were collected and lysed in RIPA buffer and the proteins were separated via SDS-PAGE.

Buffer equilibrated resin (0.5 ml) was separated and added to the cell lysate.

Aliquots of total protein, mixed in a 1 1 ratio with Laemmli buffer (Bio-Rad, Hercules, CA, USA), were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) before transfer to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, UK).