Sentence examples for buffer before being mounted from inspiring English sources

Cells were washed in Ca2 +-free Krebs buffer before being mounted into a coverslip holder (PerkinElmer, Beaconsfield, UK) and inserted into a stirred cuvette containing Ca2 +-free Krebs buffer.

Worms were incubated in 100 µl FITC conjugated WGA (20 25 µg/mL) for 1 h, washed in 1 ml M9 buffer four times before being mounted on slides for observation with Nomarski and fluorescent microscopy.

Slides were washed in PBS followed by a final wash in Tris buffered non-saline (TNS) before being mounted using fluorosave reagent (Calbiochem, Nottingham, UK).

Discs were then rinsed three times for 10 min in phosphate buffer 0.2% triton, incubated for 1 h with TRITC Donkey anti-mouse (Jackson ImmunoResearch, 715-025-150) in phosphate buffer 0.2% triton supplemented with 10% horse serum and rinsed three more times for 10 min before being mounted in Vectashield (Vector Labs, H-1000).

The sections were counterstained with hematoxylin before being mounted.

After focusing, the strips were then incubated in equilibration buffer [6 M urea, 75 Mm Tris HCl, pH 8.8, 30% (vol/vol) glycerol, 2% SDS, 1% DTT, 1% iodoacetamide] for 30 min before being mounted on a 12.5% polyacrylamide gel.

Slides were dehydrated in alcohols and cleared in three xylene baths before being mounted with permanent mounting media.

After a rinse in the same buffer, slices were mounted onto glass slides in an aqueous mounting medium.

After two final rinses in phosphate buffer, slices were mounted onto slides.

Following a series of washes in borate buffer, the slides were mounted in borate buffer for immediate imaging.

After a stringent wash with the buffer the slides were mounted with fluorescent mounting medium containing DAPI and coverslipped.