Sentence examples for buffer before being added from inspiring English sources

The resin was washed twice with 10 column volumes of lysis buffer before being added to a plastic column.

Cell extracts were diluted 1 : 20 in assay buffer before being added to the fluorescent substrate as experimentation showed this to be the optimum dilution to minimise the effect of the extraction buffer itself on the fluorescent BODIPY® substrate.

Reduced PDI (5 μM) was equilibrated in 100 mM Tris HCl, 150 mM NaCl, pH 7.5 degassed buffer before being added to samples containing roGFP variants in the reduced state followed by oxidized glutathione (100 μM; Sigma).

The labeled yeast were washed twice with 50 mM Na2HPO4 and twice with 17 mM KH2PO4/Na2HPO4 buffer, pH 6.0 (PB), before being added to Dictyostelium cells.

DNA from faeces was isolated with the QIAamp® DNA Stool Mini Kit (QIAGEN, Ontario, Canada) according to the manufacturer's instructions, with the following two modifications: i) instead of 180 220 mg, from 200 to 500 mg of the superficial faecal material was used per sample, and ii) the ASL buffer was heated to 70°C before being added to the samples.

Immunoprecipitated complexes were washed three times with 0.2 ml of RIPA buffer, and once in PBS, before being added to, and boiled in, 1× NuPAGE® sample reducing agent (Life Technologies), ready for western blotting or mass spectrometry analysis.

Pellets were resuspended in hypotonic buffer for 10 min before the addition of sample buffer, whilst sample buffer was added directly to all supernatant fractions.

Briefly, 20% brain homogenate was incubated with pK and detergent solution for 10 min at 37°C before buffer B was added.

The beads were precipitated by centrifugation steps and washed five times in NP-40 buffer before sodium dodecyl sulfate loading buffer was added.

To reduce RNA degradation the LMD procedure never exceeded one hour before extraction buffer was added to the microdissected cells.

Virions were initially concentrated from the plasma or serum by centrifugation at 21,000×g for 75 min. All but 140 µl of the supernatant was removed before lysis buffer was added.