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Following sample injection, the loop was washed with an additional 1.0 ml of buffer before being taken offline.
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Lysis Buffer and reaction Buffer were taken as the blank control.
For the staining of β-galactosidase, cells were fixed with 3% formaldehyde and 0.025% glutaraldehyde (Sigma) for 5 min, and incubated with citrate buffer (pH 5.2) containing FeCN for 2 to 16 hrs before images were taken.
Both types of GUVs were filled with buffer containing the fluorophore ATTO 647 (blue) and treated with 150 µM CLR01 for the indicated times before images were taken by confocal microscopy.
ThT was added to a 10 μM Aβ42 sample (50 mM phosphate buffer pH 7.4) to a final concentration of 20 μM, gently vortexed, and allowed to bind for 3 minutes before a reading was taken.
In contrast, intradermal or intramuscular needle injection induces less cell damage, and tissue nucleases are diluted by relatively large quantities of injection buffer so that the injected DNA can be taken up by cells before its extracellular degradation.
Samples were taken before and after laccase treatment from a membrane reactor (initial concentration of reactants: 0.1 mM, volume: 10 l; phosphate buffer; incubation time: 4 h).
**No measurement was taken before the scan.
Soil samples were taken before transplanting.
Informed consent was taken before interviewing.
Radiographs were taken before hardware removal.
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