Sentence examples for buffer before being transferred from inspiring English sources

After incubation, the reaction mixture was made up to 100 µl with binding buffer before being transferred to a streptavidin-coated 96-well plate and incubated at 37°C for 1 h.

The cells were then washed twice with 0.5 M NaCl in binding buffer before being transferred to fresh tubes for counting using a microbeta scintillation counter (LKB Wallac; Perkin Elmer, Cambridge, UK).

Cultures were acclimated to the presence of 3.75 mM buffer in TAPS-containing media before being transferred into media containing HEPES.

It was decapitated between cervicals three and four, and then fixed in 10 % Neutral Buffered Formalin (NBF) for two weeks before being transferred into an enclosed jar containing 600 mL 2.97 % (w/v) iodine-based buffered formalin solution for staining.

All tissues and cell pellets used during the validation were fixed in Neutral Buffered Formaldehyde as specified (Table 3) before being transferred directly to 70% ethanol for no longer than 3 days.

C. elegans was grown axenically in a chemically defined nutrient medium before being transferred to minimal buffer containing a standardized density of bacteria in multiwell plates.

All samples were subsequently washed twice in PBS-T at RT for 10 min before being transferred to blocking buffer (BB, 2% sheep serum, 2 mg/ml BSA in PBS-T) for minimally 1 hour at RT while on a 3D rocker.

After electrophoresis for 2 h at 95 V, the gels were then incubated for 45 min at room temperature in a solution (50 mM Tris-Cl pH 7.6, 10 mM CaCl2, 20 mM NaCl, and 1 uM ZnCl2) containing 2.5% Triton X-100 twice, before being transferred to activation buffer (50 mM Tris-Cl pH 7.6, 10 mM CaCl2, 20 mM NaCl, 1 uM ZnCl2) for overnight incubation at 37°C.

On completion of the behavioural testing, the rats were killed by CO2 asphyxiation, and the brains removed and fixed in 4% fresh paraformaldehyde in 0.1 M phosphate buffered saline for at least 48 hours before being transferred to 20% sucrose in PBS solution for cryoprotection.

Fishes were deeply anaesthetised with tricaine methanesulphonate MS-222 in seawater (500 mgl-1) and were fixed in 7% buffered seawater formalin for approximately 2 weeks before being transferred to 70% ethanol for storage.

Deuterated cADO assay buffer was prepared in 99.8% D2O with 100 mM D-HEPES (pD 6.8) and 100 mM KCl. Proteated and deuterated buffers were purged with nitrogen for 2 h before being transferred to an anaerobic chamber (Coy Laboratory Products Inc., Grass Lake, MI), where they were allowed to equilibrate for at least 0.5 h before being used.