Sentence examples for buffer before being boiled from inspiring English sources

DYNAbeads was then washed three times with 500 μL immunoprecipitation buffer before being boiled in 25 μL SDS sample loading buffer (2×).

The agarose beads were washed for five times with lysis buffer before being boiled in SDS loading buffer.

The beads were then washed thoroughly with 1× binding buffer before being boiled in 1× SDS sample buffer.

Proteins were precipitated in methanol, dried and solubilized in Laemmli sample buffer before being boiled, migrated on SDS-PAGE gel and transferred to PVDF membranes.

The final chromatin pellet was resuspended in Laemmli buffer and sonicated before being boiled and analyzed by SDS-PAGE and immunobloting.

All protein samples were diluted to 5 mg/ml before being boiled in SDS sample buffer for gel loading.

Phosphotransferase reactions were stopped by the addition of 4X sample buffer and were boiled for 5 min before their fractionation through 10% SDS-PAGE.

After three washes with the buffer, the beads were boiled in SDS loading buffer at 95oC for 5 min before electrophoresis.

The washed immunoprecipitates, resuspended in Laemmli buffer, was boiled at 95 °C for 5 min before immunodetection of SAG, Bax, SARM, Bcl-2 or ubiquitin.

Before centrifugation at 10,000 g for 5 min at 4°C, the sample buffer was boiled for 5 min.

The protein concentrations were determined by Bradford assay before the lysate was boiled with loading buffer and resolved by SDS-PAGE.