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To determine lactate production, cells were serum starved for 40 minutes, in Krebs Ringer buffer, before being exposed to 5 mM glucose for 4 hrs.
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The membrane then was washed twice in wash buffer and incubated in CDP-Star agent at RT for 5 min before being exposed to photographic film (Agfa, Belgium).
Yet it took decades before Philby was exposed.
The gills are exposed before the cap is completely developed, and before the spores are mature.
Purified skeletal or cardiac myosin in HEN buffer (HEPES, EDTA, neocuproine, as in [14]) were exposed to SNO-cys.
These animals had never been exposed before.
TiO2-NPs suspensions were freshly prepared before the fish eggs were exposed.
The varying rate of amide II COG shift could be attributable to buffer diffusion as the thin layer near the bead surface was exposed to NaOH first before it could have diffused through the matrix.
"I have been exposed to security interrogations before," says Al Ryami.
The intact cornea was exposed to phosphate buffered saline and swelled with time.
The DNA sensors were exposed to a phosphate buffer solution (PBS) containing the DNA molecules.
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CEO of Professional Science Editing for Scientists @ prosciediting.com