Sentence examples for buffer before being loaded from inspiring English sources

Cell lysates (40  μg) were boiled for 5 min in Laemmli buffer before being loaded on 10% acrylamide gels for SDS-polyacrylamide gel electrophoresis.

Briefly, the lyophilized venom (1.5 g) was dissolved in 20 mL of 0.05 M sodium phosphate buffer (pH 5.8) before being loaded on an SP-Sephadex C-25 column.

After migration, the strips were equilibrated in SDS containing buffer (reduction and alkylation) before being loaded onto SDS polyacrylamide gels for separation according to molecular weight using an Ettan DALT Six Electrophoresis System (GE Healthcare).

The protein pellets were then resuspended in SDS sample buffer and boiled for 10 min before being loaded onto an SDS-PAGE gel.

Briefly, protein extracts were diluted in loading buffer (Sigma) and boiled for 2 min before being loaded into an SDS PAGE gel and transferred to a nitrocellulose membrane (Amersham).

The samples were prepared in nonreducing Laemmli buffer and heated for 10 min at 90 °C before being loaded onto the gel.

Samples were diluted in SDS buffer and heated at 70°C for 10 min before being loaded on a NuPage 10% BisTris gel (Invitrogen, Carlsbad, CA, USA).

Protein samples dissolved in the sample buffer and the marker protein were denatured at 100 °C for 5 min before being loaded onto the gel.

Fractions containing proteins were pooled and diluted 1 1 in MQ-A buffer before they were loaded on a High-Trap Heparin column (20 ml, GE Healthcare, USA) equilibrated in MQ-A buffer containing 0.2 M NaCl.

The amplification reaction was diluted 1 : 20 with the 1 × Tris-EDTA buffer before it was loaded on TaqMan Low Density arrays (Applied Biosystems) to assess the amplified genes.

The stopped-flow mixing cell and tubing were thoroughly washed and incubated overnight with PCA/PCD buffer before stopped-flow syringes were loaded with anaerobic substrate and enzyme solutions.