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Mice subjected to EPM received a double 90-interval i.v. injection of tat-cyclotraxin-B or saline buffer before being placed on the platform of the EPM and assessed for 5 min (26×5×36 cm).
Sections were washed in phosphate buffer before being placed in blocking solution (20% normal goat serum) for 60 min. Sections were then incubated in anti- c-fos primary antibody at a concentration of 1 8000 (Oncogene Research Products, Calbiochem) overnight.
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Slides were allowed to solidify for 10 min at 4°C before being placed in lysis buffer for 1 h (2.5 M NaCl, 0.1 M ethylene diamine tetraacetic acid (EDTA), 0.01 M Tris, 1% Triton X-100, and 10% dimethyl sulfoxide (DMSO), pH 10).
before being placed on standby in April.
The whole blood sample is needed to be diluted with standardized filtration buffer before it is placed on the filtration reservoir for 3 minutes sample processing time.
Gels and buffers were equilibrated at 4°C before electrophoresis, and the buffer tank was placed in an ice bath during electrophoresis to maintain the low temperature.
Gels and buffers were equilibrated at 4 °C before electrophoresis, and the buffer tank was placed in an ice bath during the electrophoresis to keep the temperature of the gel below 15 °C.
A UN buffer force was placed in the Sinai Peninsula.
The loading solution was removed and the cells were washed twice with HEPES buffer before the coverslips were placed onto a perfusion chamber and connected to a micro pump.
These buffers are placed into both RAM and virtual memory.
Terminal deoxynucleotidyl transferase (TdT) in reaction buffer (containing a fixed concentration of digoxigenin-labelled nucleotides) was applied to sections for 30 min at 37 °C, before the slides were placed in Stop/Wash buffer for 10 min.
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