Sentence examples for buffer before being incubated from inspiring English sources

Thereafter, the cells are washed once with staining buffer before being incubated in staining buffer supplemented with 5% FBS on ice until sorting is completed.

Wells were then washed three times with 200 µl IP buffer and once with 200 µl TE buffer, before being incubated with 100 µl elution buffer (25 mM Tris base, 1 mM EDTA, pH 9.8, 200 µg/ml proteinase K) for 15 min at 55°C, followed by 30 min at 75°C.

Media was aspirated and replaced with loading buffer before being incubated at room temperature in the dark for 60 min.

CHO cells were incubated 1 min at 37°C in the fusion buffer before being incubated at 39°C with pre-warmed complete F12 media.

The tumour areas and corresponding non-neoplastic gastric mucosa were scraped using a sterile needle and placed in a microtube containing extraction buffer before being incubated at 55°C for 12 h as described elsewhere (Wu et al, 2004).

Membranes were blocked in Starting Block T20 PBS Blocking Buffer (Thermo Scientific) before being incubated overnight with primary antibodies at 4°C in PBS containing 0.05% Tween20 (PBS-T).

Cell suspensions were centrifuged at 400 g for 5 min and washed twice in cold FACS buffer (0.1%BSA/0.1%NaN3/2/2 mM EDTA in PBS) before being incubated in FcBlock (Miltenyi, Germany).

Membranes were blocked for 1 h in Tris-buffered saline containing 0.05%Tween-20Tween-20and5% milk before being incubated with primary antibodies (Cell Signaling Technology, USA, 1 : 1000).

They were then rinsed three times with washing buffer, 0.1% Triton X-100 in PBS before being incubated with Alexa Fluor 488 Goat anti Rabbit secondary antibody (Invitrogen Molecular Probes, Oregon, USA) at a dilution of 1 1000 in blocking buffer for one hour at room temperature.

In experiments with inhibitor, 0.5 μL inhibitor, 5 μL NF-D1, and 4.5 μL protein in binding buffer were incubated before addition of 40 μL NF-D2.

After three 3-min washes in PBS/Tween 20 buffer (wash buffer), sections were incubated in 1% glycine for 30 min to neutralise acidic groups before being washed again in the same wash buffer.