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In this study, we have investigated the sufficiency of targeting RAS-effectors, RAF, MEK and PI3-Kinase either alone or in combination in RAS mutant lines, using an inducible shRNA in vivo mouse model system.

Five mutations were identified by TILLING of 768 mutant lines using four target genes.

The glucan chain length distribution in total leaf starch was determined for wild type and the mutant lines using fluorophore-assisted carbohydrate electrophoresis (FACE) [ 43, 44].

In order to observe the shape of leaf epidermal pavament cells, scanning electron microscopy was performed with fresh 6th rosetta leaves of wild type or mutant lines using a Hitachi TM-1000 scanning electron microscope (Hitachi High-Technologies).

Here, we report the generation of an ethyl methanesulfonate (EMS -mutagenized sorghum mutant population, phEMS -mutagenizedant linesorghume population, TILLING analysis of a subset of mutant lines using four target genes, and verification of the observed mutations with the resultant population.

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Thus, the mutant lines used in this study were coisogenic to the C57BL/6 strain.

Mutant lines used in this study were SALK_023626, and Flag605F08 for AtIPS1, SALK_101349 for AtIPS2 and SALK_071284 for AtIPS3.

Mutant lines used in this study.

All mutant lines used in the present work are in the Col-0 background.

The Arabidopsis mutant lines used in this study are listed in Table 1.

The T-DNA mutant lines used in this study were obtained from the Arabidopsis Biological Resource Center at The Ohio State University.

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