Sentence examples for mutant proteins using from inspiring English sources

This also allowed us to purify the mutant proteins using heparin sepharose, which relies on an intact heparin-binding site in the N-terminal [Daubner and Piper, 1995].

We also examined the catalytic activity of mutant proteins using the substrate LPC.

This assay will provide a means to identify drugs which can counteract the effects of mutant proteins using high throughput screens, and has the potential to monitor effects of these drugs on ER stress in vivo.

Our results indicate that the rabbit monoclonal antibody directed against H3K27M mutations specifically recognizes mutant proteins using simple IHC.

The authors should monitor membrane binding of wild type Beclin 1 and the S90A and S90E mutant proteins using cellular fractionation approaches and in vivo localization experiments.

To investigate the potential biologic relevance of the p. (Lys372del) variant, we performed an in silico prediction on the wild-type and mutant proteins using the online protein comparative modelling server 3D-JIGSAW (http://bmm.cancerresearchuk.org/~3djigsaw/).org/~3djigsaw/

This redesign led to the development of sensitive and specific pairs of mutant proteins used to sense Ca2+ in a second generation of genetically encoded Ca2+ indicators (cameleons).

In principle all mutant proteins used here should be able to bind E-Cadherin through the Armadillo repeats [67], [68], their stability and activity dependent upon mutation of residues in the N-terminus rather than an inefficiency of binding to E-Cadherin [30], [35], [69].

We spin labelled the mutant protein using the HO4120 spin label and performed preliminary EPR experiments using cw-X-band EPR spectroscopy.

This versatile model is particularly suitable to assess in vivo how mHtt toxicity can be modified by different factors that can be co-expressed with the mutant protein using injection of a mixture of LVs in mouse striatum (for a review 33).

Functional analysis of this mutant protein using an in vitro luciferase reporter assay system confirmed that the mutation abolished the enhancing effects of wild-type GATA3 on the promoter activity of the consensus GATA responsive element and that of human PTH gene.