All mutant lines generated passed through all primary phenotypic screens.
Incidence of tumors in the three Rb1 mutant lines generated by ENU mutagenesis.
In double mutant analysis the same phenotype was observed for, at least, two independent double mutant lines generated.
We resolved ENU-induced mutation screening for 8 phenotypic mutant lines generated on C3HeB/FeJ background aligning the sequences against the closely related, but not annotated reference of C3H/HeJ.
Recently, the MGP reported an infertility rate of 5.2% in 307 homozygous mutant lines generated from the IKMC targeted ES cell resource (White et al., 2013).
Here we report on our screening approach to identifying signaling components required for R gene-mediated disease resistance, using a collection of NB-background mutant lines generated by activation of the rice endogenous retrotransposon Tos17 [ 26].
The CARDIF −/− (MAVS, IPS-1, or VISA) mutant line generated by the Tschopp group in Lausanne was obtained from Caetano Reis e Sousa in London.
By screening of individual DNA samples from 4500 M2 mutant wheat lines generated by heavy ion irradiation, we detected multiple mutants with deletions of each TaPFT1 homoeologue, and confirmed these deletions using a CAPS method.
In vitro studies using CLN3 mutant cell lines generated from mouse models and human patients have suggested roles for CLN3 in many cellular processes including intracellular trafficking, endocytosis, apoptosis, autophagy and lipid cycling (reviewed in 3).
To test whether post-transcriptional repression of Med and Shn by Brat is relevant in vivo, we visualised Med protein in germaria from wild-type flies and those with brat mutant germ lines (generated by tissue-specific flipase mediated mitotic recombination).
