Sentence examples for mutant samples using from inspiring English sources

Confidence-interval analysis validated the ability to distinguish between wild-type, heterozygote, and mutant samples using the READIT Assay.

Mutation profiling was repeated on 32 mutant samples using ColoCarta and the results were identical to results with OncoCarta, demonstrating that this methodology was reproducible.

Briefly, RNA was extracted from batch cultures of mid-exponential phase (OD595 ∼ 0.3 0.6) from mutant and isogenic wild-type strains, reverse-transcribed into cDNA, and labeled with either Alexa Fluor 555 (wild-type sample) or Alexa Fluor 647 (mutant sample) using Superscript Indirect cDNA labeling system (Life Technologies).

Total RNA was prepared from both mutant and control samples using QIAGEN RNeasy kit (Valencia, CA, USA).

We analyzed differential expression between mutant and wild type samples using the R package DESeq.

Percent Clonality and Adjusted Mutant Frequencies in Control and BaP-treated samples using the LOD/linear model to adjust mutant counts.

Three biological replicates of the wild-type and the hrpX mutant samples were used for RNA-seq analysis.

Synthetic mutant samples were used for this evaluation: WT genomic DNA from clinical glioma FFPE samples was spiked in 3 mastered proportions (5, 10 and 30% mutant copies) with either R100Q, R132C, R132H or R172K plasmid mutant DNA.

Total RNA was extracted from two wild-type and two mutant samples (frozen hippocampus) using TRIzol.

Expression values were then imported into R and differential expression analysis between wild-type and mutant samples was performed using DESeq [ 47].

Differential expression of biological replicates between wild type and mutant samples was determined using DESeq [ 59], based on the number of RNA-Seq reads uniquely mapped to defined genomic loci and regions.