Sentence examples for mutant plants using from inspiring English sources

Genomic DNA was extracted from the leaf blades of two-week-old M23 and z3 mutant plants using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) according to the manufacturer's instructions.

The construct obtained was then introduced into ehy5 (hy1) mutant plants using A. tumefaciens-mediated transformation.

The binary vector was introduced into Agrobacterium strain GV3101 and transformed into mutant plants using floral dip method [ 52].

Genomic DNA was isolated from leaves of wild type and mutant plants, using DNeasy plant mini kit (Qiagen).

To generate stable transgenic lines, constructs were introduced in wild-type and spl7-2 mutant plants using Agrobacterium tumefaciens GV3101 (pMP90RK) in the floral-dip method [ 50, 51].

Genomic DNA samples were prepared from wildtype (Ler) and two independent as1-21 mutant plants using the DNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands).

The Arabidopsis wild type and mutant plants used in this study were in the Columbia (Col-0) background.

Nowadays, Ac/Ds is used as a tool in plant biology to generate mutant plants used for the characterization of gene function.

Therefore, the mutation is most likely controlled by a recessive nuclear gene.The mutant plants used in our experiments were the stable eighth generation individuals (M8) derived from the isolated original mutant plant.

Total RNA was extracted from various tissues of wild-type, mutant, and transgenic plants using MG Total RNA Extraction kit (Doctor Protein, Korea).

Thus, transcript levels of genes close to the T-DNA insertion site were compared in the mutant and WT plants using quantitative RT-PCR.