Blocking these activities using yeast mutants also disrupts substrate degradation, suggesting a role in ERAD [15], [16].
Synthesis of serine derivatives having the essential functional groups for the inhibitor of SARS 3CL protease and evaluation of their inhibitory activities using SARS 3CL R188I mutant protease are described.
Since WRN helicase is known to exhibit other enzymatic activities, such as 3'→5' exonuclease and DNA strand annealing, we tested these activities using the WRN mutant proteins.
All three Sso0660 mutants were assayed for their protease activities using the azocasein assay.
While retaining its aldehyde reductase activities using the cofactor NADH, mutant Y62-G6 disignificantlyficantly greater reductase activities using NADPH as the cofactor with 13- and 15-fold increase toward furfural and HMF, respectively, as measured by its partially purified protein.
We next assessed the impact of the Pocket 2 mutants on Parkin activity using an E2 discharge assay that measures the ability of ubiquitinPhospho-Ser65 to stimulate the discharge of the ubiquitin-loaded E2, UbcH7, by Parkin.
We have further characterised Pex10p's E3 ligase activity using mutants designed to disturb this activity and show that Pex10p acts as the E3 ligase for Ubc4p-dependent ubiquitination of Pex5p but not Pex4p-dependent ubiquitination in vivo.
In silico methodologies such as 3D-QSAR and molecular docking were performed to explore compounds with better mutant isocitrate dehydrogenase 1 (Minhibitorybitory activity using a series of 40 newly reported 1-hydroxypyridin-2-one compounds as MIDH1 inhibitors.
Moreover, we confirmed the essential role of NF-κB in C. crepidioides-induced iNOS promoter activity using deletion mutant forms of the two NF-κB recognition sites in the iNOS promoter, termed NF-κB1 and NF-κB2.
Since BRAF is not active in RAS mutant melanoma cells, we measured T529NBRAF activity using transient expression in COS cells (Wan et al., 2004).
