Sentence examples for mutant constructs using from inspiring English sources

The underlined nucleotides were introduced into the mutant constructs, using the QuickChange Mutagenesis kit.

The cells were transfected with the nSMase1 mock, wild-type, S270A mutant, and S270E mutant constructs using TransIT Jurkat Transfection Reagent (Mirus Bio LLC, Madison, WI, USA).

293T cells were cultured on glass coverslips and transfected with Myc-BRCA1 wild-type and mutant constructs using Fugene 6 (Roche, Melbourne, Australia) according to the manufacturer's instructions.

At DIV5, neuron cultures were transfected with DARPP-32-wild-type and DARPP-32-T153A mutant constructs using Lipofectamin 2000 (#11668-019; Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.

Rat pheochromocytoma cells (PC12) cultured in DMEM supplemented with 10% horse serum and 5% FCS, were transiently transfected with the WT or either of the 2 mutant constructs using lipofectamine 2000.

MEL cells expressing BirA biotin ligase (Schuh et al., 2005 ) were transfected with pEF1α-bioSCL or pEF1α-bioLMO2 (biotin-tagged, wild-type and mutant constructs) using Transfast (Promega) and selected in Zeocin (200 μg/ml, Invitrogen) or G418 (750 μg/ml; GIBCO).

The structures of PAPC and FLRT3 as well as mutant constructs used in this study are shown in Figure S1.

MA provided the initial concept, and the ezrin mutant constructs used in the study.

We decided to focus our analysis on KLF9 uAUGs since the effects of wild-type and mutant constructs used to elucidate the roles of uAUGs were demonstrated in both cell-lines relevant to tissue specificity.

All mutant constructs used here (p31(comet), MAD2, and TRIP13) expressed at levels similar to wild-type, and migrated equivalently on a size-exclusion column (not shown), indicating that they were soluble and folded.

The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2407/9/229/prepub We thank the members of the 2006 Graduate Genetics laboratory course for their help in creating some of the VHL mutant constructs used in this study.