Sentence examples for mutant strains using from inspiring English sources

Figure 2 Average 3H4MV fraction in PHA synthesized by R. eutropha PHB- 4 expressing phaC1 Ps (parent strain) and leucine analog resistant mutants (mutant strains) using MS plus fructose (20 g/L) medium.

We chose to generate our own mutant strains using the same parental wild-type as there may be differences unrelated to the mutation between strains obtained from other labs or a culture collection.

A preliminary assay of five rpl5 mutant strains using the near- (AGC) and non- (UCU) cognate reporters indicated that the K27E mutant of L5 tended to be generally hyperaccurate (data not shown).

Unfortunately we were not able to detect HrcUXAC in the wild-type or complemented mutant strains using the anti-HrcUXAC polyclonal antibodies in this study (data not shown) and so could not determine relative levels of HrcUXAC in the Xac strains nor have we so far been able to determine whether HrcUXAC is in fact cleaved at the NPTH site in Xac cells.

Biofilm formation in different media was assessed for both WT and ΔtolC mutant strains using the crystal violet biofilm assay.

Briefly, genomic DNA was isolated from WT and mutant strains using a commercial kit according to the manufacturer's instructions (Mini-bacterial DNA isolation kit, Tiangen Biotech).

Wild-type and mutant strains used originate from the Bristol strain N2 [31].

The wild-type strain used was Canton S. The mutant strains used was palets1 (plets1, a temperature-sensitive TH allele) [14].

Each of the mutant strains used in this work were created using allelic exchange, resulting in markerless deletions (Figure 1).

However, it was proposed that the observed defects could be an indirect consequence of the particular mutant strains used [21].

The wild type C. elegans N2 and tnt-3(aj3) mutant strains used in this study were obtained from the Tan Laboratory at Stanford University.