Sentence examples for mutant libraries using from inspiring English sources
However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible.
Quality evaluation of the mutant library using PCR specific for the kanamycin resistance gene in the Tn5 transposon.
Based on the results of our analyses of adhesion and invasion, the strain SAKA10119 was selected to construct a mutant library using the EZ-Tn5™ < KAN-2 > Transposon.
In the current study, the adhesive and invasive capabilities of 56 C. sakazakii strains against human epithelial cells were evaluated, and one of them was selected for construction of a mutant library using the Tn5 transposon.
In the present investigation we generated a saturated mutant library using pSAM_Bt and identified the genes required for the survival of B. fragilis.
To identify histone H3 and H4 residues genetically connected to TORC1 signaling, we screened a histone H3/H4 mutant library using a sub-inhibitory (25 nM) concentration of rapamycin to identify those mutants exhibiting altered rapamycin sensitivity.
To elucidate the role of TORC1 signaling in chromatin regulation, we screened a budding yeast histone H3 and H4 mutant library using the selective TORC1 inhibitor rapamycin to identify histone residues functionally connected to TORC1.
The mutant library used was an isogenic library of inbred tomato cultivar M82 derived from ethyl-methane sulfonate (EMS) mutagenesis treatment.
Finally, a novel D-3-hydroxybutyrate short-chain dehydrogenase with less than 35% similarity to known proteins with similar activity has been isolated from soil metagenomic libraries using a mutant of Sinorhizobium meliloti that was unable to synthesize D-3-hydroxybutyrate [ 32].
This interactome is of high quality because the database was constructed of multiple screens of 9 cDNA libraries using wildtype and mutant forms of G protein baits.
The HA plasmid mutant libraries were used to generate pools of mutant influenza viruses by reverse genetics (Hoffmann et al., 2000).
