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To test whether AKHR plays a role in the regulation of CREB activity, we examined whether CRE-mediated transcription is altered in AKHR mutant flies using the CRE-Luciferase reporter transgene.

By blocking chemical transmission in shak-B mutant flies using tetanus toxin we can deduce that the chemical component is functional in the absence of gap junctions.

(A ) Courtship index comparing CS control and miR-124 mutant flies using decapitated CS or miR-124 mutant males as targets.

Mitochondrial free radical production and oxygen consumption in dj-1β mutant flies We measured mitochondrial free radical production in 10 day old dj-1β mutant flies using flies from the DAH background as controls.

To do so, the human or the hamster GPHR proteins were expressed a in dGPHR mutant flies using a ubiquitous Gal4 driver (daughterless-Gal4) and the weight of the emerging adult flies was used as a readout of body size.

Similar(53)

One possibility is that the apparent reduction in life span and vigor previously reported for p53 null flies may have resulted from inbreeding depression in the homozygous mutant flies used in that study.

Finally, we analyzed respiration rates in mitochondria isolated from mutant and control flies using NAD+-linked respiratory substrates (pyruvate plus proline) that deliver electrons into complex I of the mitochondrial electron transport chain.

The wildtype control and mutant cyc01 flies used here are in the same y w genetic background and were treated contemporaneously.

Furthermore, we observed living larval body wall muscles in tw mutant flies by using the MHC-tauGFP reporter, a muscle marker (Figs. 3F H).

We provide a detailed description of the pattern and morphology of the direct flight muscles in the wild type and lms mutant flies by using the recently-developed ultramicroscopy and show that, in the mutants, all DFMs are present and present normal morphologies.

Cuticular hydrocarbon profiles were generated for sexually mature miR-124 mutant and control male flies using gas chromatography/mass spectrometry (GC-MS).

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