Sentence examples for mutant variants using from inspiring English sources

The ability of the Drosophila transgenes to successfully rescue vps35-mutant viability allowed us to further test the relative functionality of the mutant variants using the climbing assay.

(B ) PIC formation on the SSV1-T6 promoter in response to TFEα/β, TFEα and mutant variants using EMSAs.

This specificity "switch" was further confirmed by performing phosphorylation assays on both the wild-type and mutant variants using a pair of matched peptide substrates of identical sequence save for having Ser or Thr in the P0 position.

We next determined the kinetic parameters of the Trp114 mutant TrxR1 variants using well-characterized TrxR1 substrates.

To test whether the reduced helicase activity of Twinkle adPEO variants impaired mtDNA replication in a similar way, we analyzed the mtDNA replication pattern of cultured cells expressing wild-type and mutant Twinkle variants using two-dimensional DNA gel electrophoresis (Fig.  6).

Using ketosteroid isomerase as a model system, we correlate the field sensed at the bond involved in enzymatic catalysis with the rate of the reaction it catalyzes, including variations in this rate in a series of mutants and variants using non-canonical amino acids.

As shown in Fig. 3G I, all mutant TDP variants used in our study (D169G, G298S, A315T and N345K) exhibited a significantly higher eccentricity and compactness compared with D42 driver controls.

The strategy we report here offers a single step for efficient site-saturation combinatorial mutagenesis of multiple positions using Gibson Assembly and productive sampling of the mutant library (>1 million variants) using positive antibiotic selection.

We compiled a global set of publicly available somatic cancer mutations from COSMIC v67 (Forbes et al., 2011) and generated the FASTA files required by ReKINect containing both the wild-type and mutant versions of all coding missense variants, using purpose-made Python scripts and ENSEMBL's VEP resource (Flicek et al., 2014).

A panel of nine Gβ1 mutant variants was used to benchmark the screen, and spectroscopic methods were employed to characterize the thermal and structural properties of each variant accurately.

For functional analysis of the mutant variants, we used a heterologous hyper-expression system, where GFP-tagged CaMdr1p (CaMDR1-GFP) wastablyly over-expressed from a genomic PDR5 locus in AD1-8u-, a S. cerevisiae mutant.