Sentence examples for mutant forms using from inspiring English sources
To assess the functional role and physiological importance of the cytoplasmic gate we investigated the functionality of hAQP10 and mutant forms using protepolymersome- (in vitro) and S. cerevisiae-based (in-vivo) assays (Supplementary Figs. 3b and c 29.
We have overexpressed and purified wild type VR1 (wtVR1) as well as several mutant forms using the yeast strain Saccharomyces cerevisiae with the goal of obtaining sufficient protein for structural studies.
We first determined expression of the myc-tagged mutant forms using western blot analysis of embryo lysates.
The phosphorylation of all Aurora A samples including mutant forms used here were quantitatively confirmed by mass spectrometry (MS).
In this study, we applied this approach to HeLa cells and then systematically examined the role of individual anti-apoptotic Bcl-2 proteins and their phospho-mutant forms, using untagged full-length proteins that closely mimicked the properties of their endogenous counterparts.
Our server automatically generates conformational ensembles for both wild-type and mutant-type forms using RosettaBackrub [24], and performs docking simulations of given drugs to both types using GOLD [25] and AutoDock Vina [26].
Notch mutant forms were generated using the Stratagene site-directed mutagenesis kit and primers with appropriately altered amino acid codons.
HEK29T cells grown in 10-cm dish were transfected with pcDNA3.1-HA-SOCS6, pcDNA3-c-myc-ubiquitin, and native or mutant forms of YAP1 using calcium phosphate.
The T254A and T254C mutant forms were produced using the primers 5′-CGTGGTGACCACC GCGACCCACAAGACGC-3′ and 5′-CGTGGTGACCACC TGCACCCACAAGACGC-3′, respectively, and their complementary oligonucleotides (the mutated codons are underlined).
Finally, functional analysis using mutant forms based on human KRT5 mutations demonstrated the potential to use the chicken as a model to study keratin-based diseases.
The nra gene of the classic skin strain Alab49 (M protein type 53, M53; FCT-3 region form) was chromosomally replaced with the rofA gene from the classic throat strain D471 (M protein type 6, M6; FCT-1 region form), using the Δnra mutant as a recipient (Figure 1A B).
