Sentence examples for mutant seedlings using from inspiring English sources

After optimization, stress was imposed on 21-day-old mutant seedlings using 25%% (w/v) PEG6000 for 6 days.

About 2 g of 10-day-old try cpc double mutant (separate root and leaf) and gl2-2 mutant seedlings were cross-linked using 1% formaldehyde solution.

For micrografting experiments four-day-old Arabidopsis thaliana wild type and hen1-1 mutant seedlings were cut transversely using a sterile small razor blade part and combined within silicon tubing (0.3 mm internal diameter) as previously described [ 50].

Additional analyses of cell wall composition of hypocotyls of dark-grown Col, hsr8-1, hsr8-1pft1-2 and pft1-2 mutantseedlingsgs were conducted using Fourier Transform InfraRed spectroscopy (FTIR) [ 19].

To identify trafficking routes regulated by BIG1-4, pathway-specific soluble and membrane-associated cargo proteins were analysed in BFA-treated wild-type and big3 mutant seedlings (for a list of markers used, see Supplementary file 1; Figure 2 figure supplement 1S,T).

The double mutant plants were back-crossed successively to wild-type (WS) and the segregated ehy5 mutant seedlings in the F2 generation was selected and used for further back-crosses with wild type (WS) for four generations (to purify the background mutations in the EMS treated hy5-ks50 mutant plants) before physiological and genetic analysis.

To evaluate the function of BZR1-ARF6-PIF4 interegulating regeneting gexpressionsion, we carried out RNA-Seq analyses using wild type and the iaa3/shy2-2 mutant seedlings treated with mock or BL for 4 hr.

For the experiment in Figure 6, seedlings grown in liquid culture were used as the membrane source [ 15]; the double mutant seedlings were initially selected on Petri dishes based on phenotype then transferred to liquid culture.

At the fifth true leaf stage, stem apexes of wild type and mutant seedlings were harvested and immediately frozen in liquid nitrogen and stored at −80 °C until further use.

In contrast, mutant seedlings that lacked AFP took much longer to start growing again.

However, root length measurements of rlk902 rkl1-1 and rlk902 rkl1-2 double mutant seedlings revealed a slight but significant enhanced reduction in root length compared to rlk902 mutant seedlings (Figure 1b).