Sentence examples for mutant alleles using from inspiring English sources

Here, we investigated the function of Cftr during zebrafish development by generating several cftr mutant alleles using TAL effector nucleases.

A PCR amplification reaction targeting the deletion breakpoint in normal, heterozygous and homozygous mutant individuals with the Filipino deletion was modified to detect simultaneously the normal and mutant alleles using three PCR primers 45F: 5' TCA GAA GCA GAG CTA CTC AG 3'; 45R1- 5' GTC TAT GCA GGT GTG TAG ACA 3' and 45R2: 5' CAT TTA GCT CCC ACA CTC CT 3'.

Therefore, we repeated our analysis of sspA-S47 mutant alleles using strains harboring a deletion for sspB.

A simple PCR-based assay was developed, based on the differential amplification of the normal and mutant alleles using a trio of primers.

Our method could be applied to other cereal crops that have similar waxy mutant alleles using primers that are similar to those used in this study.

To examine the function of Gpr124 during zebrafish development, we generated two gpr124 mutant alleles using TAL effector nucleases (Cermak et al., 2011; Dahlem et al., 2012).

All mutant alleles used in this study are well characterised.

Mutant alleles used in genetic complementation tests are listed in Table S4 (crosses that complemented) and Table S5 (crosses that failed to complement).

Transgenes and mutant alleles used in this work were as follows: Mad-flag wild type UAST transgene on chromosome 3 was yw Bl/Cyo MWT/MWT, and on chromosomes 2 and 3 yw MWT/MWT MWT/MWT with Cyo and TM6B floating.

Mutant alleles used are described in Table S1.

All mutant alleles used in this study either eliminate or severely reduce gene function.