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Previously, we have shown that phenotypic and splicing defects observed in an sr45 knockout mutant were rescued by SR45 fused to GFP [24], indicating that SR45-YFPC fusions used in this study are functional.
Changes in the alternative splicing of SR genes and the phenotypic defects in the mutant were rescued by SR45 cDNA, further confirming that the observed defects in the mutant are due to the lack of SR45.
> -wrap-foot> Flowering time, siliques/seed production and dark phenotypes of cvy1 mutant were rescued by 35S CVY1 gene expression in a complementation test.
As observed for morphology, Akt activation and apoptosis, the reduced levels of proliferation observed in the mutant were rescued by re-expression of GFP-tagged OCRL1 (Fig. 7C).
The impaired growth and defects in glycogen synthesis of the glc7-E101Q mutant were rescued by introduction of a single copy of wild type GLC7, indicating that glc7-E101Q is a recessive, hypomorphic allele.
Similar(54)
By providing the viruses with the opportunity to increase their fitness by mutation, a mutant was rescued that found a way to compensate for the imposed fitness cost.
A second mutation that renders UDG defective in nucleotide flipping was then introduced, and the double mutant was rescued using a substrate with a "preflipped" cytosine base.
By using our established reverse genetics system for BVDV, we confirm that the viral mutant is rescued through a −1/+2 ORF initiated in the Npro(1 19)/eGFP region of the mutant viral genome.
The Or42a − mutant was rescued with Or42aGAL4-UAS- Or42a; the Or42b − mutant was rescued with Or42aGAL4-UAS- Or42b.
Indeed, the clearance defect in the psr-1 mutant was rescued by overexpression of human PSR.
Further, the ECPC phenotype of spectrin mutant is rescued by dampening MLC activity.
Related(20)
mutant were found
mutant were analyzed
mutant were obtained
mutant were grown
mutant were overexpressed
mutant were isolated
mutant were analysed
mutant were performed
mutant were done
mutant were cotransfected
mutant were used
mutant were soaked
mutant were selected
mutant were produced
mutant were normalized
mutant were generated
mutant were expressed
mutant were cloned
mutant was rescued
mutant were interpreted
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