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To determine the impact of minimizing phycobilisome antenna size through deletion of the apcE gene on photoautotrophic growth, GT and the ∆apcE mutant were grown under atmospheric air conditions.
Importantly, we detected additive defects when delg mutant were grown under low yeast (Fig. 6A).
SH1000 and the SH1000 sigS mutant were grown in competitive culture experiments as described previously by Doherty et al [83].
Y. pestis WT strain 201 and the Δhfq::KmR mutant were grown in LB medium at 26°C overnight.
Two independent cultures of each, the wild type strain and the phoP mutant were grown until OD600≈0.45.
Both the wild-type and hfq mutant were grown with empty pKK214 vector to account for any plasmid maintenance effects in the complemented strain.
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Mutant and wild-type bacteria were grown in pure culture and each mutant was grown in mixed culture with the wild type, with a starting frequency of 50%, in ASM.
Expression of OsPT1 was induced only when osnla1 mutant was grown under Pi-deficient condition.
A culture of the P. stutzeri A1501 mutant was grown overnight in LB broth supplemented with kanamycin at 30 °C.
To determine the reaction of G978-18 to panicle blast, the mutant was grown to maturity under natural conditions in Cavinti, Laguna, Philippines, known to be a "hotspot" for blast.
Since ESA1 is essential, the temperature-sensitive esa1 mutant was grown at nonpermissive temperatures and samples were prepared.
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