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The constructs used to create each mutant were isolated by PCR (primer sequences available upon request).
The cells were lysed 24 hr later and Ptch1 and its mutant were isolated with anti-FLAG agarose beads and resolved by SDS-PAGE on 6% or 4 12% gradient gels.
Similar(58)
The gns4 mutant was isolated from EMS-treated japonica variety Zhonghua 11C (ZH11C).
A short root rice mutant was isolated by screening a T-DNA insertion library [64].
The smg11 mutant was isolated from the japonica variety Kuanyejing (KYJ) mutagenized with EMS.
The HTS semi-rolled leaf mutant was isolated from an ethyl methane sulfonate (EMS -induced japonica ricEMS -induced bank.
This improved biocatalyst was engineered further and a new mutant was isolated that was capable of the same performance with 5-fold reduction in biocatalyst loading.
The small grain 11 (smutantmutant was isolated from the ethyl methanesulfonate (EMS -treated M2 populations of thEMS -treatedariety Kuanyejing (KYJ).
First, to increase the de novo GTP supply, an 8-azaguanine resistant mutant was isolated and an additional guaBA operon was introduced.
A rice heading-date-related mutant was isolated from a 60Co-γ-ray-induced mutation pool of Zhejing 22, a conventional japonica cultivar in Zhejiang Province, China.
The zebra3 1 (z3–1) mutant was isolated from a mutant pool produced by applying N-methyl-N-nitrosourea, to a japonica rice cultivar 'Kinmaze', as previously described (Iwata et al. 1979).
More suggestions(15)
mutant were associated
mutant were analysed
mutant were exposed
mutant were stained
mutant were obtained
mutant were cloned
mutant were assayed
mutant were transfected
mutant were purified
mutant were cultured
mutant were performed
mutant were grown
mutant were compared
mutant were rescued
mutations were isolated
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