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Atm+/+ (WT) and Atm−/− mESCs were obtained from oocytes of Atm+/− mice17, and NSG mESCs (Prkdc mutant) were obtained from NSG mice68.
Five biallelic mutants, two heterozygous mutants and one homozygous mutant were obtained from 16 independent transgenic hairy root lines when the sgRNA was driven by the Arabidopsis U6 promoter, while no mutants were obtained from 13 independent transgenic hairy root lines when the sgRNA was driven by the rice U3 promoter.
Genomic DNA samples from ES clones stably expressing an RFM mutant were obtained after three weeks of continuous cell culture.
The ctx::km and ctx::kmΔprtV mutant were obtained by phage-mediated transduction using the CP-T1ts phage [29].
High stringency datasets for the Nrl mutant were reported previously [25], and low stringency datasets for the Nrl mutant were obtained as described above.
pCMV5-Flag-LKB1WT wild type, pCMV5-Flag-LKB1KD kinase dead, pCMV5-Flag-LKB1S431A mutant and pCMV5-Flag-LKB1S431D mutant were obtained from Dario Alessi (University of Dundee).
If several amplified fragments are visible and no mutant is obtained, increase the annealing temperature.
The rice mutant was obtained from KDML 105 rice embryos bombarded with N+ + N2+ ions at an energy level of 60 keV and ion fluence of 2 × 1016 ions/cm2.
The ghd10 mutant was obtained from japonica cultivar Wuyunjing 7 (WYJ7) rice plants subjected to EMS treatment.
The edd − mutant was obtained from the UW P. aeruginosa Mutant Library [15].
Microarray dataset for Dam mutant was obtained from Robbins-Manke et al [3].
