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The recombinant full length PARP2 or the NTR deletion mutant were generated and examined in the in vitro PARylation assays.
Mutants on the ribH1, ribH2 genes and a double ribH mutant were generated.
Mutants on the two lumazine synthases (LS) isoenzymes RibH1 and RibH2 and a double RibH mutant were generated.
For transfection, TC45-WT and TC45-D182A/Q262N substrate-trapping mutant were generated as described before [22], [23].
RAW264.7 cells stably expressing the very same Flag-tagged TIAR transgene or a RNA-binding defective TIAR mutant were generated and were used for RIP-ChiP analysis.
The gcr2-4 gcl2-1 double mutant and gcr2-4 gcl1-1 gcl2-1 triple mutant were generated by crossing gcl2-1 single mutant with gcr2-4 gcl1-1 double mutant and isolated in the F2 progeny by PCR genotyping.
Similar(40)
M. smegmatis ΔnucS (MSMEG_4923, nucSSm) in-frame deletion mutant was generated by allelic replacement37.
After three rounds of mutagenesis, a mutant was generated that decreased the biocatalyst loading 3-fold.
The pGL3-basic luciferase vector (Promega, Madison, WI, USA) was used to clone the promoter of TLR4, and LSD1-binding site-specific mutant was generated by PCR.
A model of the N51A mutant was generated by truncating the N51 side chain to transform it into an A residue.
viciae AapQ site directed mutant was generated as follows.
More suggestions(15)
mutations were generated
variants were generated
mutant were found
mutant were analyzed
mutant were obtained
mutant were overexpressed
mutant were grown
mutant were isolated
mutant were analysed
mutant were performed
mutant were done
mutant were used
mutant were cotransfected
mutant were soaked
mutant was generated
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