Sentence examples for mutant were cloned from inspiring English sources

The plasmids that complemented the temperature sensitivity of the apm1-1 mutant were cloned and sequenced.

For transgenic analyses, the five truncations of MKS1 and the MKS1-L32A point mutant were cloned into the NotI site of the binary vector pJL12 using primers listed in Table S2.

The cDNAs of the rBos d 5 B and rBos d 5 B H146P mutant were cloned into the bacterial expression vector (pKKtac) and transformed into E. coli RV308 strain.

The PCR products, either wt or mutant, were cloned into P1 pAltermax vector and plasmids sequenced.

Mouse HA-tagged HOIP WT and CS (C693S/C696S) mutant were cloned into pTY-EF1A-hygromycinR lentiviral vector (see below).

The first 300 amino acids of the human FBW7 protein and the T205A mutant were cloned into the pGEX-4T-1 vector to generate bacterial expression constructs.

To investigate it, the deletion mutant was cloned in the bicistronic plasmid construct (Fig. 5A).

In the next step, His-tagged ftsZMTB (or its mutant) was cloned under the control of the hsp60 promoter in pUC19 using KpnI and HindIII sites as described above.

Ng-IQless mutant was cloned from the pEGFP-Ng plasmid by PCR in which amino-acids 30 45 were removed.

Each single mutant was cloned into pET28b and pCIneo mammalian expression vector (Promega Corp., Madison, WI, USA).

As the genes corresponding to each mutant are cloned, a more sophisticated comparison of the steps in eudicot and grass anther development can be conducted.