Sentence examples for mutant were done from inspiring English sources

Both the proteins of WT and spl5 mutant were done 2-DE for 3 gels, respectively.

Using this cDNA as a template the nucleotide substitutions of the H146P mutant were done by a PCR cloning.

Mutagenesis of the SA0746-encoded nuclease gene (designated here as nuc1) in UAMS-1 and its isogenic sarA mutant was done using pKOR1 as previously described [7].

The Nck1 mutant was done on the SH3 domain by changing the first tryptophan (W) residue in the conserved WW motif to lysine (K) [ 19].

Expression and purification of wild-type AfUGM and all mutants were done under conditions previously reported.

Expression, purification, and spin labeling of the mutants were done as described in the Supporting Information.

The genotyping and selection of homozygous triple mutants were done similarly, using the primers listed in supplementary material Table S1.

Identification of the positive transformants that were homozygous for the T-DNA insertions resulting in the irx9-2, irx7, and irx8-6 mutants were done as described by [ 33].

Secretory mammalian expression in HEK293T cells and purification via affinity chromatography of EGb-Ki4 scFv) EGb-Ki4 scFvnts were done anddescritsd previously.

Here, we must note that the measurements for the cathodic peaks of both mutants were done by a mathematical deconvolution (detailed explanation appears in the Experimental Section) due to reductive catalytic peaks that appeared in more negative potentials probably due to bacterial lysis on the surface.

Expansion of Cer1 and Foxa2 in Itga5 mutants was done on a 129S4 background.