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Field trials for the OsINV3 WT and the mutant were performed to test their agronomic and growth characteristics.
Kinetics experiments with the wild type NTD and the K216A mutant were performed under similar conditions.
All statistical analyses for cul1, cul3 and slmb mutant were performed using Student's t-test.
Separate mixed plate matings between each donor strain, CDC-A3580s1 (pBotCDC-A3-Erm), 657BaCT4 (pCLJ-Erm), and Eklund 17BCT11 (pCLL-Erm) and recipient strains LNT01 and Hall A-hyper/Tn916 mutant were performed inside an anaerobic chamber on solid 4% agar TYG media for 12 h.
Further biochemical and molecular characterizations of the mutant were performed on these progeny plants.
To show that Ufd2 stabilization role upon Yap8 is independent of its proteolytic function, various assays using the Ufd2 U-boxΔ mutant were performed.
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Genotyping of the T-DNA insertion mutant was performed by Southern and PCR analysis.
Genetics analysis of ral1 mutant was performed by using an F2 population from a backcross between ral1 and its wild type Kasalath.
The R85A mutant did not express and no further analysis of this mutant was performed.
Construction of the tpx mutant was performed using a two-step mutagenesis strategy [30].
The selection of the tpx mutant was performed as described previously [31].
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