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Briefly, M3 seeds of each candidate mutant were soaked in deionized water for 2 d at 37 °C.
Crystals of the inactive E217Q mutant were soaked with the tetrasaccharide substrate analogues 5 and 6 resulting in two separate complexes (MltC· 5 and MltC· 6) each showing excellent electron density and occupying the −1 to −4 subsite positions.
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Surface-sterilized seeds of el1 mutant and WT plants were soaked in water for 7 days and then placed in soil and grown in a greenhouse.
Teguments of imbibed mutant and wild-type seeds were torn and embryos were soaked in a 1% 2,3,5-triphenyl tetrazolium chloride solution (Sigma, CA).
For mutant library construction, seeds of an Al-intolerant indica cultivar (cv. Kasalath) were soaked in tap water for 8 h at room temperature.
They were soaked.
Her penny loafers were soaked.
People were soaked in there".
We were soaked.
Bundles were soaked in running water overnight.
They were soaked now.
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