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Both sequences of the PCR product amplified from the mutant were analysed by DNA sequencing to confirm the deletion of the tpx gene (data not shown).
Ten cells of each mutant were analysed.
Randomly 25 fibres from each set (control and mutant) were analysed.
Transcript levels of AtMYB80 in both wild-type and atmyb80 mutant were analysed using real-time qRT-PCR.
Samples from leaf patches agroinfiltrated with the ΔGDD TuMV mutant were analysed using the same primers as above.
To test whether the chromosomal deletions altered the fitness of the strains, the growth patterns of each mutant were analysed over 24 h in CFA and LB medium.
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Following the generation and transfection of expression vectors carrying each mutation, the HBA2 transcription activity of the promoters from each mutant was analysed with quantitative real time-PCR (qReTi-PCR) technique.
Each mutant was analysed in triplicate.
The overall dynamics of each mutant was analysed by comparing the calculated B-factors from each simulation.
The ability of LF82 OMVs to restore the invasion of the LF82-Δ ompA isogenic mutant was analysed.
To investigate the contribution of NcGRA17 α-helices to aberrant morphology of PVs, we transfected four truncated forms of NcGRA17 into NcGRA17 knockout strain and the phenotypes of these mutants were analysed.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com