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The binding affinity displayed by these compounds positions them as lead compounds for the design of future inhibitors of heat shock protein 90 based on the β-lactam and imine templates.
The ability of LigB-12 to retain the binding affinity displayed by LigBCen2R for Fg was investigated using ELISA.
These analyses led to the observation that the binding affinity displayed by the various NF-κB dimers for distinct κB sites does not necessarily correlate with what occurs during regulation of gene expression by NF-κB in vivo.
In this proof-of-concept study we show that CBMs indeed have the potential to be utilized in the characterization of chemically modified celluloses and can, due to the selective binding affinity displayed by the individual CBM, be used as tools to make a distinction between homogeneously and heterogeneously substituted MC and HPMC.
We could not exclude the formal possibility that the albeit rather small increase in Crm1 binding affinity displayed by the PKI, MAP and Rev NES as compared to the AID NES might explain the inability of these heterologous NES to potentiate CSR.
Similar(55)
Moreover, our results clearly indicate that there is indeed a hierarchy in terms of preferential incorporation of proteins into the nanoparticle scaffold, a preference that is dictated by the differential binding affinities displayed by the proteins themselves.
A couple of small molecule inhibitors of the ZipA FtsZ interaction have been developed to show that binding affinities displayed by the FtsZ peptide can potentially identify new drugs disrupting the PPI (Fry, 2006).
That multiple proteins should bind to carbonate HAP used in these experiments is consistent with the well-known protein-binding affinity displayed by both this mineral and calcium [51].
As this mutant binds a single chitin molecule with high affinity (kd = 4.95 nM), we concluded that the ultra-high chitin-binding affinity displayed by wild-type Ecp6 corresponds to the LysM1 LysM3 binding groove.
It was also postulated that the conserved motifs lying outside the DNA binding domain may also contribute to the differential affinity displayed by ERF proteins towards the GCC box-containing promoters.
This system has been employed to evaluate the affinity for the Kef ligand binding domain displayed by a range of natural and synthetic GS-X analogues.
Related(19)
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