Possible causes for the large CV of TETN include: (i) the binding affinity varied between different depletion columns and/or (ii) the binding affinity was sensitive to the loading volume.
However, the binding affinities varied according to the order: f-ImPy∗Im (2) > f-ImPyIm (1) ⩾ f-Im∗PyIm (5) confirming that the second amino group can improve affinity, but its position within the polyamide can affect affinity.
In Arabidopsis, secondary wall NAC family proteins (SWNs) activate their direct target genes through binding to the SNBE sites, and the binding affinities vary with different SWNs and SNBE sequences (McCarthy et al., 2014; Zhong et al., 2010).
The similarities between the two cytokine profiles were unexpected because the binding affinities vary significantly.
Its binding affinity varies from picomolar to nanomolar and is independent of the sequence and the structure of DNA ends, but it is affected by the length of DNA duplex [ 24– 27].
The binding affinities can vary by several orders of magnitude [ 13].
While the affinity of binding between E111 and DIII varied by genotype, a limited correlation with neutralizing activity was observed.
The binding mechanisms and affinities vary also between epothilones (Khrapunovich-Baine et al, 2011).
Plasma concentrations of HP, and its binding affinities for free Hb, vary by phenotype, with HP22 reported to have reduced overall Hb affinity (Langlois & Delanghe, 1996), less efficient antioxidative capacity, (Langlois & Delanghe, 1996; Melamed‐ Franket al, 2001) and reduced clearance rates of HP2‐Hb complexes by macrophages (Asleh et al, 2003).
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
