Exact(3)
Top-3,000 Top-3,000 Top-3,000ted from the protein-protein doconformations were selectedd to energy minimizations, and the docked structures were ranked according to the binding afromitheestimated by using the Molecular Mechanics/protein-proteinnn Surface Area (MM/PBSA) methodocking
The Fab Ala mutants were expressed as phage and the relative binding affinity estimated by competition ELISA.
The binding affinity estimated by solid-phase assay with recombinant protein forms might not necessarily be the same as the affinity to the native structure forms on the cell surface; thus, the equivalency in anti-CD20 efficacy was observed.
Similar(57)
The RNA-binding affinity estimated by C50 values of full-length JAZ (ZF1 4) is higher than that of one- or two-finger constructs.
We attempt to approximate its sequencing preference by the binding affinity estimated from applying PDNN on the nucleotide sequences nearby, which represents how strong a single-stranded DNA sequence can bind to its template sequence.
In this study, the binding affinity was estimated by using an implicit-solvent model in which the electrostatic contributions were obtained by solving the Poisson equation, and the hydrophobic effects were accounted for by using surface-area-dependent terms.
Kd values and relative binding affinities were estimated by using a 5-fold dilution series of protein, from the in vitro transcription-translation system.
The binding affinities were estimated by monitoring changes in the native tryptophan fluorescence of the V domain upon titration with MG-Hs (Table 1 and Figure S2 of the Supporting Information).
Typically, ligand-binding affinity is estimated by scoring functions.
For direct recovery of high-affinity-binding aptamers, the resin of the affinity column was physically segmented into slices and the amount of retained binding aptamers was estimated by quantitative real-time PCR.
The binding affinities were estimated as a Docking score (Glide score + Epik state penalties).
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