Also, the finding that a p73 mutant in the DNA-binding domain displays no apoptotic activity in Saos-2 cells,72 is in accordance with this model.
Remarkably, while Akt1 PH domain displayed strong binding affinity to PtdIns 3,4 P2 and PtdIns 3,4,5 P3 (together referred to as PIP2/3), there was no binding to these lipids by the PH domain of Cla4.
Future work will determine whether this newly identified region in XopD and/or the KAE-rich domain, related to dimer formation, determine the specificity of the DNA binding activity displayed by the XopD protein.
Vinculin −/− cells expressing the head domain displayed a higher binding strength than those expressing the tail domain.
Hrk_ΔTM and Hrk_medium show significant levels of interaction, with the entire cytosolic domain displaying higher binding affinity (Fig. 1B).
As shown in Figures 7C and 7D, whereas isolated full-length VAMP7 showed little binding to Hrb, participation in a SNARE complex restored Hrb binding of the VAMP7 cytoplasmic domain to levels comparable to that displayed by the isolated longin domain.
Two chimeras containing different Cys2 His2 domains displayed highly sequence-specific binding and regulation.
Domain and localization analyses of CoAtg26 show that both the phosphoinositide binding and sterol glucosyltransferase activities displayed by this enzyme are required for infection-related pexophagy.
The specific TFs of bacteria and archaea are dominated by DNA-binding domains (DBDs) displaying different versions of the helix-turn-helix (HTH) fold, several of which were already present in common ancestors of the two superkingdoms [ 3].
This effort involved the construction and screening of 3 distinct libraries: a domain library that included several engineered binding domains isolated by phage-display; a lower-resolution linker library; and a higher-resolution linker library.
