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Aptamers with high binding affinity, reflected by low nanomolar Kd values, are sought for use in bioassays and in eventual clinical application.
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Moreover, it has been directly demonstrated that peptide binding affinity reflects peptide transport affinity [30], [31].
We found a wide spectrum of binding affinities, reflecting known genetic interactions.
As expected from the EMSA experiments, illumination results in an increased DNA binding affinity of PtAu1afull, reflected by a pronounced protection of the basic DNA binding region and a decrease in structural dynamics of leucine zipper peptides.
Any sequence variation may change its protein binding affinity, which is reflected by a change in the computed Ri of that binding site.
As the method is semiquantitative, the different intensities between the amounts of P1 and P2 precipitated by ZF + KTS may reflect differences in binding affinity or may reflect technical variation.
Low affinity reflects binding to uncoupled receptors.
However, both TSAd Tyr and Tyr are phosphorylated by Lck to a similar extent in vitro [ 21], thus the difference in amount of TSAd mutants pulled down could also reflect differences in binding affinity, as indicated by SMALI (Fig. 1b).
The reciprocal influence of the binding of A on the affinity of B and the binding of B on the affinity of A is reflected by γ = γ+/γ−.
Thus, surface plasmon resonance (SPR) biosensor analysis was used to test direct binding of WA to HRas-GTP/GDP protein, and the binding affinity of WA to the proteins was reflected by response unit (RU) values.
Moreover, the binding affinity of WB to the proteins was reflected by response unit (RU) values.
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