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Nonspecific DNA decreased helical structure of ArntbHLH-C/EBP, an observation difficult to explain; however, this might explain the weaker binding affinity exhibited by ArntbHLH-C/EBP for the E-box.
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However, the trend in the loss of chaperone activity of α-crystallin does not parallel the trend of binding affinities exhibited by these peptides for this chaperone.
To understand the molecular basis for the unexpected drug binding affinities exhibited by the QacR(E57Q) and QacR(E58Q) mutants, we conducted X-ray crystallographic experiments with these QacR mutants in complexes with Be, Dq, and MG.
Highest AR binding affinity exhibits dihydrotestosterone (DHT), which is ten times higher compared with testosterone.
The higher affinity Mcl-1 binding exhibited by the peptides makes them better Mcl-1-targeting reagents than the previously engineered Mcl-1-specific peptides or the natural BH3 NoxaA.
This ligand exhibited improved binding affinity predicted by means of free energy calculations.
We measured the O2/CO partition coefficient and the kinetics of oxygen dissociation and carbon monoxide binding and found that lowered O2 and CO affinity is also exhibited by the R-state tetramers and the isolated beta chains of Hb Chico.
Although these variants exhibited significantly reduced steady-state rate constants that decreased monotonically with increasing residue bulk, CTAD binding affinity was unaffected by mutation.
Remarkably, the T14 (FG) chimera exhibited only a slightly lower affinity for Ebola GP, while, both the T14 (BC) and T14 (chimerasmeras decreased the binding affinity by 10 folds (Table 1 and Fig. S5).
Because distinct mutants of PML-RARA exhibited different AS2O3 binding affinity and degradation pattern followed by AS2O3 treatment, we next detected whether these mutants affect the basal SUMOylation of PML-RARA.
The ZnCar MOF exhibits strong binding affinity for both gases, as shown by the isosteric heat of adsorption Qst.
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