Sentence examples for binding affinity measured by from inspiring English sources
While CETSA relies on an irreversible thermal aggregation of the target protein, and is therefore not a system at equilibrium, we attempted to derive an apparent intracellular binding affinity, measured by quantification of target engagement within live cells.
We identify that the global stabilization of GALC conferred by azasugar derivatives, measured by fluorescence-based thermal shift assays, is directly related to their binding affinity, measured by enzyme inhibition.
This indicated that the binding affinity measured by gas-phase method of ESI-TOF-MS is comparable to that acquired by solution-phase method.> The binding of commercial telomerase inhibitor TMPyP4 tetratosylate with human telomeric DNA G-quadruplex was examined by ESI-TOF-MS technique in this study.
Based on the binding affinities measured by ITC and by NMR, the binding free energies differ by −0.89 ± 0.04, 0.23 ± 0.09, and 0.71 ± 0.08 kCal/mol upon E64RP mutation, for the bindings towards tUIM, NZF, and ZnF4, respectively.
Similar to several previous studies [ 4, 10], for computing PCC, the binding value was obtained by 1 - log IC50)/ log 50, 000), where IC50 is binding affinity measured in nM.
The correlation coefficient r indicates the level of correlation between the binding affinity predicted by our models and the affinity measured by biochemical experiments.
NECA had a binding affinity as measured by H-DPCPX whole-cell binding of 1 µM.
A typical binding curve for [F]FAN-128 is presented in Figure 2. The binding affinity was measured by determining EC50 and slope from this experimental titration curve (22).
(h) Granulosa cells were harvested for ChIP analysis and the FoxO3a binding affinity was measured by real-time PCR assay.
This value represents the intracellular equivalent of an apparent binding affinity as measured by inhibitory constant, Ki, and is determined using a cellular target engagement CETSA HT assay following compound incubation with prostate-cancer derived cells endogenously expressing AR.
PrimPol DNA binding affinity was measured by incubating p/t-DNA substrates (1 nM) labeled at the 5′-end of the primer with FAM with varying concentrations of PrimPol (0 to 4 μM).
