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In order to identify those five optimal lines, scientists first had to create hundreds of mouse stem cell lines.
When I vetted that estimate with Martin Evans, a biologist in Wales who is the elder statesman of mouse stem cell research, he agreed that it was in line with his work on mice.
Expression of stem cell transcription factors was performed using Mouse Stem Cell Transcription Factors RT2 Profiler PCR Array.
But now, his group has found a mouse stem cell that behaves like human stem cells do.
High expression of BC markers (KRT5, P63, NGFR and S100A) and another putative mouse stem cell marker integrin α6β4 (Chapman et al., 2011) were observed in clones.
But a new technique may finally be bringing that process into the open: A team of Chinese researchers reports turning a dish of a mouse stem cell into spermlike cells, which then were used to fertilize eggs and produce healthy mouse pups.
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Aggregation turns embryonic mouse stem cells into mice.
However experiments in which dopamine neurons were created from mouse stem cells have not been successfully reproduced in humans.
Scientists at the University of Pennsylvania transformed mouse stem cells into eggs in 2003, but did not use them to create mouse pups.
But Gage notes that immature neurons derived from mouse stem cells are more active than their mature counterparts, so an extended maturation period may actually be beneficial.
Using mouse stem cells arranged in a three dimensional culture, Dr Sasai's team mimicked the way pituitary glands develop in the embryo.
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