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Overexpression of 17β-HSD13 17β-HSD13e hepatocyte cell line induced liver steatosis and lipid accumouseion.
African green monkey kidney epithelial cells (Vero-E6), HEK293T, and a mouse hepatocyte cell line (Hepa 1 6) were cultured in DMEM supplemented with 10% FBS (Gibco).
Herein, we demonstrate for the first time that fenofibrate induces the expression of LDLR mRNA and protein in a mouse hepatocyte cell line (AML12 cells) and that the induction of LDLR expression enhances LDL uptake.
However, Kim et al. [ 40] noted that the viability of an uninjured mouse hepatocyte cell line treated with BV did not change significantly, although this was most likely due to the minute concentration used (10 ng/mL).
To study the effects of fenofibrate on hepatic LDLR expression, a mouse hepatocyte cell line, AML12 cells, was treated overnight with different concentrations of fenofibrate in serum-free medium.
Hepa1-6 cells (mouse hepatocyte cell line) were cultured in a humidified atmosphere at 37°C with 5% CO2, and grown in DMEM (Dulbecco's modified Eagle's medium) containing 10% (v/v) heat-inactivated fetal bovine serum, 25 mM glucose, 100 U/ml penicillin, and 100 mg/ml streptomycin [ 35].
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Mouse hepatocyte cells AML 12 (ATCC, Manassas, VA,USA) were grown at 37°C in Dulbecco's modified Eagle's medium/Ham's F-12 nutrient mixture with 10% fetal Bovine serum, 100 nM dexamethasone and ITS (insulin, transferrin and selenium, Invitrogen, Burlington, ON, Canada).
The gene expression profiles observed by complementary DNA (cDNA) microarray showed that HBx can lead to aberrant expression of molecules involved in signal transduction, transcriptional regulation, protein degradation, cell cycle control, apoptosis, metastasis, immune response and metabolism in mouse hepatocyte cells (18).
The mouse hepatocyte AML12 cell line was purchased from ATCC.
To further confirm this observation, we transfected HBx expression plasmid into mouse hepatocyte AML12 cells and examined its effect on mEZH2 expression.
For mouse hepatocytes, the cell suspension is transferred into a single 50-ml falcon tube.
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