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This ligand receptor interaction is known to play important roles in mouse germ cell migration and proliferation.
Isolation of mouse germ cell types is a well established approach, used to obtain microarray gene expression data for specific germ cell types [18].
We provide evidence that DNA adducts induced in male germ cells persist in spermatozoa and that they give rise to de novo mutations, also demonstrating B[a]P as a mouse germ cell mutagen.
It has also been described that TU-1A is regulated by an alternative promoter sequence [6], [8] that proved to be very active when transfected in the GC2 mouse germ cell line [7].
This suggests that the function of Nanos3-3'UTR is relatively unique in the mouse germ cell, unlike fly and nematodes in which 3'UTRs of many genes each have significant responsibility for the temporal and spatial control of a specific protein in their germlines [4], [35].
Worldwide, opportunities for the costly mouse germ cell studies are limited.
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Related sites Hans Schöler Azim Surani's group studying mouse germ cells.
Isolated mouse germ cells were treated with different concentrations (0.05, 0.5 and 1 mM, respectively) of Dox, and fixed 1 h after treatment.
The specific aim was to assess the relative importance of the postulated mechanisms in generating deletions in the human genome and examine whether empirical data on radiation-induced deletions in mouse germ cells are consistent with predictions of these mechanisms.
The present investigation was designed to determine whether exposure of isolated and purified mouse germ cells to Dox induces DNA damage in the form of strand breaks (presumably) resulting in apoptosis and to investigate the relative sensitivity of specific cell types.
Apart from miRNAs, mouse germ cells express another type of small RNA, piwi-interacting RNAs (piRNAs).
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