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The increase in cellular ROS level were also detected in the HMF-exposed primary cultures of mouse muscle cell (Fu et al., 2016).
We also extended these studies to differentiated primary mouse muscle cell cultures; we observed two types of myotubes in primary cultures: thin slowly contracting myotubes with nuclei aligned along the middle of the fiber (immature myotubes), and thicker, faster contracting myotubes often with small or large nuclei clusters (mature myotubes).
We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling.
We also verified AMPK phosphorylation of NADSYN1 by using C2C12 cells, which are a mouse muscle cell line.
It has been reported that Wnt agonist promoted mouse muscle cell proliferation, and specific silencing RNA knockdown of Wnt 4 significantly reduced muscle cell proliferation [ 38].
The C2C12 mouse muscle cell line (ATCC number: CRL-1772) is an accepted cell system for the study of cellular mechanotransduction [ 35].
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By inactivating two genes that work to suppress tumors, they got mouse muscle cells to revert to a younger state, start dividing and help repair tissue.
The Stanford team shut off both Rb and Arf with a chemical called silencing-RNA and found the mouse muscle cells started dividing.
The researchers then took a number of the metabolites that had been elevated by exercise and infused them into mouse muscle cells in a laboratory dish.
Furthermore, Nip30 and Rspry1 were transcriptionally induced in response to neurogenic muscle wasting in mice and were also found to be expressed endogenously at the RNA and protein level in C2C12 mouse muscle cells.
A range of gene delivery vectors containing the thermoresponsive polymer, poly N-isopropylacrylamide) (poly N-isopropylacrylamideeffects on cell viability, intracellular trafficking and transgene exPNIPAmon in C2C12 mouse muscle cells.
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